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KMID : 0364819860240010018
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Korean Journal of Microbiology
1986 Volume.24 No. 1 p.18 ~ p.23
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Characterization of the Restriction Endonuclease Bdi Ⅰfrom Brevibacterium divaricatum
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Kim Yong-Sok
Rho Hyune-Mo
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Abstract
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1
A new type II restriction endonuclease, Bdi I, has been isolated from Brevibacleriunt dit´aricatunt FERM 5948 by procedures of ammonium sulfate fractionation, DEAE-cellulose chromatography and heparin agarose chromatography. The purified Bdi I restriction endonuclease had the same cleavage patterns of Cla I whose recognition sequence is 5´ ATCGAT 3´. From the result that A-Cla I DNA fragment could be cloned in pBRR322 digested with Bdi I, it has been proven that Bdi I cuts between T and C (5´ AT / CGAT 3´) within the recognition sequence and produces 5´ pCG cohesive end. The optimal temperature for the Bdi I restriction endonuclease activity was 37°C, and optimal salt (NaCl) concentration was 50-100 mM.
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KEYWORD
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